Author:
Singh Lakhvir,Hu Linghao,Walsh Alex J.
Abstract
A cost friendly and compact frequency domain fluorescent lifetime microscope (FD-FLIM), with the potential of capturing lifetimes with resolution of a few nanoseconds, has been theorized and designed using detection signal processing to encode the phase shift of fluorescence emission within the intensity captured via camera. Model simulations of the system parameters illustrate the calibration process and effectiveness of the technique. The results will facilitate the development of a novel FD-FLIM that uses simple optical components and a conventional camera for improved modularity, ergonomics, software-interface, precision control, automation, compactness, and cost-effectiveness over traditional lifetime microscopes.