Affiliation:
1. Ramona Optics Inc.
2. Duke School of Medicine Department of Neurobiology
3. Duke University
Abstract
This paper experimentally examines different configurations of a
multi-camera array microscope (MCAM) imaging technology. The MCAM is
based upon a densely packed array of “micro-cameras” to
jointly image across a large field-of-view (FOV) at high resolution.
Each micro-camera within the array images a unique area of a sample of
interest, and then all acquired data with 54 micro-cameras are
digitally combined into composite frames, whose total pixel counts
significantly exceed the pixel counts of standard microscope systems.
We present results from three unique MCAM configurations for different
use cases. First, we demonstrate a configuration that simultaneously
images and estimates the 3D object depth across a 100×135mm2 FOV at approximately
20 µm resolution, which results in 0.15 gigapixels (GP)
per snapshot. Second, we demonstrate an MCAM configuration that
records video across a continuous 83×123mm2 FOV with twofold increased resolution
(0.48 GP per frame). Finally, we report a third high-resolution
configuration (2 µm resolution) that can rapidly produce
9.8 GP composites of large histopathology specimens.
Funder
Office of Research Infrastructure
Programs, National Institutes of Health
NIH Office of the Director
National Institute of Environmental
Health Sciences
National Cancer Institute
National Institute of Biomedical Imaging
and Bioengineering
National Science Foundation
Duke Coulter Translational Partnership
Award
Subject
Atomic and Molecular Physics, and Optics,Electronic, Optical and Magnetic Materials
Cited by
4 articles.
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