Affiliation:
1. Huazhong University of Science and Technology
2. Chinese Academy of Sciences
3. Hainan University
4. Optics Valley Laboratory
5. Advanced Biomedical Imaging Facility
Abstract
Acquiring the firing sequence of neurons located in varying depths with
cellular resolution is essential to understand the underlying
functional neural circuits of living animals. Light-sheet fluorescence
microscopy (LSFM) enables neuronal activities recording with high
temporal and spatial resolutions. However, constrained by the
single-plane illumination and detection configuration, neurons located
in varying depths are impossible to be recorded simultaneously. To
address this challenge, we develop the simultaneous multi-plane
imaging light-sheet fluorescence microscopy (SIMPLE-LSFM) by combining
multi-plane or region-of-interest illumination of a sample, and
spherical-aberration-assisted point spread function in detection space
for simultaneous imaging of different depth within a sample.
SIMPLE-LSFM is demonstrated by acquiring neuronal activities of six
depths simultaneously in the larval zebrafish; while keeping cellular
resolution, millimeter-scale field of view, and
>
100
H
z
temporal resolution. Firing sequences
of the neurons located in varying depths were successfully captured.
The SIMPLE light-sheet methods enable high-speed cellular-resolution
multi-depth-resolved mapping of anatomic structure and fast dynamics
in three dimensions.
Funder
The Shanghai Pujiang Talent
Plan
Major Special Science and Technology
Project of Hainan Province
National Natural Science Foundation of
China
The Innovation Project of Optics Valley
Laboratory
Subject
Atomic and Molecular Physics, and Optics,Electronic, Optical and Magnetic Materials
Cited by
6 articles.
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