Diet supplementation formulated with Bacillus sp. SMIA-2 and its enzymes for Nile tilapia: zootechnical performance and effects on intestinal morphometry-Reference-Cited by-同舟云学术

Diet supplementation formulated with Bacillus sp. SMIA-2 and its enzymes for Nile tilapia: zootechnical performance and effects on intestinal morphometry

Published:2023-12-13 Issue: Volume:46 Page:e64312
ISSN:1807-8672
Container-title:Acta Scientiarum. Animal Sciences
language:
Short-container-title:Acta Sci. Anim. Sci.

Author:

Pierro Priscilla Cortizo Costa,Mendonça Pedro Pierro^ORCID,Santos Paola de Oliveira^ORCID,Pardo Thayna de Souza,Assis Thaianara Galdino do Nascimento^ORCID,Silva Samuel Oliveira da Silva^ORCID,Ribeiro David Carvalho dos Santos^ORCID,Martins Meire Lelis Leal^ORCID

Abstract

The use of probiotics and exogenous enzymes in fish feed is a promising alternative to improve animal performance. This study evaluated the feasibility of applying Bacillus sp. SMIA-2 and its enzymes as supplements in the diet for juvenile tilapia. The effect of adding different concentrations of Bacillus sp. SMIA-2 and its enzymes in food on zootechnical development, intestinal morphometry of animals was analyzed. The bacteria could be recovered from the intestines of animals, demonstrating its ability to survive gastric and bile acids. The comparative study of SMIA-2 with commercial products showed a significant effect on individual food intake, final weight and weight gain in all treatments. Gut length, villus height and intestinal coefficient were an advantage of SMIA-2 compared to commercial products and the control group. Therefore, the inclusion of Bacillus sp. SMIA-2 and its enzymes in fish feed may represent a viable alternative to improve animal development and significantly increase intestinal villi, contributing to nutrient absorption and animal health.

Funder

Fundação de Amparo à Pesquisa e Inovação do Espírito Santo

Publisher

Universidade Estadual de Maringa

Reference37 articles.

1. Andrade, M. V. V. D., Delatorre, A. B., Ladeira, S. A., & Martins, M. L. L. (2011). Production and partial characterization of alkaline polygalacturonase secreted by thermophilic Bacillus sp. SMIA-2 under submerged culture using pectin and corn steep liquor. Food Science and Technology, 31, 204-208. DOI: https://doi.org/10.1590/S0101-20612011000100031

2. Bernardo, S. P. C., Rosana, A. R. R., de Souza, A. N., Chiorean, S., Martins, M. L. L., & Vederas, J. C. (2020). Draft genome sequence of the thermophilic bacterium Bacillus licheniformis SMIA-2, an antimicrobial-and thermostable enzyme-producing isolate from Brazilian soil. Microbiology Resource Announcements, 9(17), e00106-20. DOI: https://doi.org/10.1128/MRA.00106-20

3. Carvalho, R. V. D., Corrêa, T. L. R., Silva, J. C. M. D., Viana, A. P., & Martins, M. L. L. (2008). Otimização das condições de cultivo para a produção de amilases pelo termofílico Bacillus sp. e hidrólise de amidos pela ação da enzima. Food Science and Technology, 28(2), 380-386. DOI: https://doi.org/10.1590/S0101-20612008000200017

4. Castillo, S., & Gatlin, D. M. (2015). Dietary supplementation of exogenous carbohydrase enzymes in fish nutrition: A review. Aquaculture, 435, p. 286-292. DOI: https://doi.org/10.1016/j.aquaculture.2014.10.011

5. Cornélio, F. H. G., Cargnin-Ferreira, E., Borba, M. R. D., Mouriño, J. L. P., Fernandes, V. A. G., & Fracalossi, D. M. (2013). Crescimento, digestibilidade e resistência à infecção por patógeno em tilápia-do-nilo alimentada com probióticos. Pesquisa Agropecuária Brasileira, 48(8), 863-870. DOI: https://doi.org/10.1590/S0100-204X2013000800008