Affiliation:
1. Lomonosov Moscow State University
2. Institute of Biomedical Chemistry (IBMC)
Abstract
An electrochemical immunosensor based on screen-printed graphite electrodes has been developed for the determination of the antibiotic chloramphenicol in water and milk samples. It has been shown that the immobilization of chloramphenicol-specific antibodies in a liquid crystal layer of a membrane-like didodecyldimethylammonium bromide preserves the mobility and accessibility of active centers of antibodies, and the addition of gold nanoparticles improves electron transfer from the electrode surface to the redox centers of horseradish peroxidase used as a label. The limit of detection of chloramphenicol in water was 0.02 μg/L, in milk - 0.04 μg/L. The method can be used to determine residual amounts of chloramphenicol in animal products.
Funder
Lomonosov Moscow State University