Correlation of MCM2 Detection with Stage and Virology of Cervical Cancer

Author:

Amaro Filho Sérgio Menezes12,Nuovo Gerard Jerry3,Cunha Cynthia B4,De Oliveira Ramos Pereira Luiza1,Oliveira-Silva Michelle5,Russomano Fábio6,Pires Andrea7,Nicol Alcina F1

Affiliation:

1. Laboratory of Interdisciplinary Medical Research (LIPMED), IOC, Fiocruz, Rio de Janeiro - Brazil

2. Genetics Division, Brazilian National Cancer Institute (INCA), Rio de Janeiro - Brazil

3. Comprehensive Cancer Center, Ohio State University, Columbus, Ohio - USA

4. Department of Infectious Disease of Evandro Chagas Clinical Research Instituto, IPEC, Fiocruz, Rio de Janeiro - Brazil

5. Oncovirology laboratory, Brazilian National Cancer Institute (INCA), Rio de Janeiro, Brazil and IOC, Fiocruz, RJ - Brazil

6. National Institute of Women, Children and Adolescents Health Fernandes Figueira (IFF), Fiocruz, Rio de Janeiro - Brazil

7. Fonte Medicina Diagnostica Laboratory and UFF, Niterói - Brazil

Abstract

The highly conserved mini-chromosome maintenance proteins (MCM) are important in the initiation of DNA replication. Few studies have correlated MCM expression with the progression of cancer. Objectives (i) To analyze the expression of MCM2 in cervical cancer; (ii) to correlate MCM2 expression with the clinical tumor staging according to FIGO classification, and (iii) to relate HPV type to MCM2 expression. Methods Tissue micro-arrays (TMA) from patients with invasive cervical cancer and controls were analyzed. Human papillomavirus (HPV) DNA detection and HPV types were determined by in situ hybridization, PCR, and sequencing. MCM2 expression was analyzed by immunohistochemistry. Results The most prevalent HPV types found in invasive cancer were HPV 16 (66.6%), followed by HPV 33 (11.8%), and HPV 35 (3.6%). An increased (p<0.05) expression of MCM2 was found in invasive cervical cancers compared to controls. Moreover, a strong correlation was found between the MCM2-positive cells and the presence of HPV DNA detected by in situ hybridization. No statistically significant difference was observed between MCM2 expression and FIGO stage. Conclusions The present study shows that HPV-infected cells strongly express MCM2; nevertheless, our data suggests that MCM2 is not a good biomarker when comparing the different clinical stages of cervical cancer.

Publisher

SAGE Publications

Subject

Cancer Research,Clinical Biochemistry,Oncology,Pathology and Forensic Medicine

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