Protocol for editing fibroblasts with in vitro transcribed Cas9 mRNA and profile off-target editing by optimized GUIDE-seq
Author:
Funder
Helse- og Omsorgsdepartementet
Norges Forskningsråd
Academy of Finland
Barncancerfonden
Publisher
Elsevier BV
Subject
General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience
Reference6 articles.
1. Rapid genome editing by CRISPR-Cas9-POLD3 fusion;Reint;Elife,2021
2. GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases;Tsai;Nat. Biotechnol.,2015
3. Single-Step qPCR and dPCR Detection of Diverse CRISPR-Cas9 Gene Editing Events In Vivo;Falabella;G3 (Bethesda, Md.),2017
4. A qPCR method for genome editing efficiency determination and single-cell clone screening in human cells;Li;Sci. Rep.,2019
5. Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing;Miyaoka;Sci. Rep.,2016
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