Protocol for precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas-Reference-Cited by-同舟云学术

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Protocol for precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas

Published:2024-03 Issue:1 Volume:5 Page:102774
ISSN:2666-1667
Container-title:STAR Protocols
language:en
Short-container-title:STAR Protocols

Author:

Nievergelt Adrian Pascal,Diener Dennis Ray,Bogdanova Aliona,Brown Thomas,Pigino Gaia^ORCID

Funder

European Molecular Biology Organization

Horizon 2020

Horizon 2020 Framework Programme

European Research Council

Human Frontier Science Program

Deutsche Forschungsgemeinschaft

Publisher

Elsevier BV

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

Reference9 articles.

1. Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas;Nievergelt;Cell Reports Methods,2023

2. Preparation of Gamete Autolysin-Improved Protocol - Chlamydomonas Resource Center;Waffenschmidt,2005

3. Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9;Doench;Nat. Biotechnol.,2016

4. A Streptomyces rimosus aphVIII gene coding for a new type phosphotransferase provides stable antibiotic resistance to Chlamydomonas reinhardtii;Sizova;Gene,2001

5. Nourseothricin N-acetyl transferase (NAT), a new selectable marker for nuclear gene expression in Chlamydomonas;Yang;Plant Methods,2019

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