Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
Author:
Publisher
Springer Science and Business Media LLC
Subject
Biomedical Engineering,Molecular Medicine,Applied Microbiology and Biotechnology,Bioengineering,Biotechnology
Link
http://www.nature.com/articles/nbt.3437.pdf
Reference54 articles.
1. Jinek, M. et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816–821 (2012).
2. Mali, P. et al. RNA-guided human genome engineering via Cas9. Science 339, 823–826 (2013).
3. Cong, L. et al. Multiplex genome engineering using CRISPR/Cas systems. Science 339, 819–823 (2013).
4. Jinek, M. et al. RNA-programmed genome editing in human cells. eLife 2, e00471 (2013).
5. Hartenian, E. & Doench, J.G. Genetic screens and functional genomics using CRISPR/Cas9 technology. FEBS J. 282, 1383–1393 (2015).
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