Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9-Reference-Cited by-同舟云学术

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

Published:2016-01-18 Issue:2 Volume:34 Page:184-191
ISSN:1087-0156
Container-title:Nature Biotechnology
language:en
Short-container-title:Nat Biotechnol

Author:

Doench John G,Fusi Nicolo^ORCID,Sullender Meagan^ORCID,Hegde Mudra,Vaimberg Emma W,Donovan Katherine F,Smith Ian,Tothova Zuzana,Wilen Craig,Orchard Robert,Virgin Herbert W,Listgarten Jennifer^ORCID,Root David E

Publisher

Springer Science and Business Media LLC

Subject

Biomedical Engineering,Molecular Medicine,Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

Link

http://www.nature.com/articles/nbt.3437.pdf

Reference54 articles.

1. Jinek, M. et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816–821 (2012).

2. Mali, P. et al. RNA-guided human genome engineering via Cas9. Science 339, 823–826 (2013).

3. Cong, L. et al. Multiplex genome engineering using CRISPR/Cas systems. Science 339, 819–823 (2013).

4. Jinek, M. et al. RNA-programmed genome editing in human cells. eLife 2, e00471 (2013).

5. Hartenian, E. & Doench, J.G. Genetic screens and functional genomics using CRISPR/Cas9 technology. FEBS J. 282, 1383–1393 (2015).

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