A new vector coupling ligation-independent cloning with sortase a fusion for efficient cloning and one-step purification of tag-free recombinant proteins
Author:
Funder
University of QueenslandUniversity of Queensland (UQ)
Publisher
Elsevier BV
Subject
Biotechnology
Reference20 articles.
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2. A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site;Stols;Protein Expr. Purif.,2002
3. Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins;Arnau;Protein Expr. Purif.,2006
4. Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent;Hochuli;Nat. Biotechnol.,1988
5. Self-cleaving fusion tags for recombinant protein production;Li;Biotechnol. Lett.,2011
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