Application of a Novel CL7/Im7 Affinity System in Purification of Complex and Pharmaceutical Proteins

Author:

Chow Louise T.,Vassylyev Dmitry G.

Publisher

Springer US

Reference42 articles.

1. Khairil Anuar INA, Banerjee A, Keeble AH, Carella A, Nikov GI, Howarth M (2019) Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox. Nat Commun 10(1):1734. https://doi.org/10.1038/s41467-019-09678-w

2. STRATEGENE (2017) Affinity® Protein Expression and Purification System and Affinity® Protein Expression Vectors. https://www.chem-agilent.com/pdf/strata/204300.pdf. Accessed 28 Dec 2021

3. Gerace E, Moazed D (2015) Affinity pull-down of proteins using anti-FLAG M2 agarose beads. Methods Enzymol 559:99–110. https://doi.org/10.1016/bs.mie.2014.11.010

4. PROMEGA (2016) HaloTag® Protein Purification System Technical Manual. https://www.promega.com/resources/protocols/technical-manuals/0/halotag-protein-purification-system-protocol/. Accessed 28 Dec 2021

5. Schmidt TG, Skerra A (2007) The strep-tag system for one-step purification and high-affinity detection or capturing of proteins. Nat Protoc 2(6):1528–1535. https://doi.org/10.1038/nprot.2007.209

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