Molecular cloning of a cDNA encoding chicken T-protein of the glycine cleavage system and expression of the functional protein in Escherichia coli. Effect of mRNA secondary structure in the translational initiation region on expression.
Author:
Publisher
Elsevier BV
Subject
Cell Biology,Molecular Biology,Biochemistry
Reference28 articles.
1. Hydrogen carrier protein from chicken liver: Purification, characterization, and role of its prosthetic group, lipoic acid, in the glycine cleavage reaction
2. Purification and characterization of chicken liver T-protein, a component of the glycine cleavage system.
3. Mechanism of the glycine cleavage reaction. Steady state kinetic studies of the P-protein-catalyzed reaction.
4. Mechanism of the glycine cleavage reaction. Further characterization of the intermediate attached to H-protein and of the reaction catalyzed by T-protein.
5. Mechanism of the glycine cleavage reaction. Properties of the reverse reaction catalyzed by T-protein.
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1. Dimethylsulfoniopropionate-Dependent Demethylase (DmdA) from Pelagibacter ubique and Silicibacter pomeroyi;Journal of Bacteriology;2008-12-15
2. Crystal Structure of Human T-protein of Glycine Cleavage System at 2.0Å Resolution and its Implication for Understanding Non-ketotic Hyperglycinemia;Journal of Molecular Biology;2005-09
3. Robustness, evolvability, and neutrality;FEBS Letters;2005-02-05
4. Analyzing and enhancing mRNA translational efficiency in an Escherichia coli in vitro expression system;Biochemical and Biophysical Research Communications;2004-05
5. Probing the H-protein-induced Conformational Change and the Function of the N-terminal Region of Escherichia coliT-protein of the Glycine Cleavage System by Limited Proteolysis;Journal of Biological Chemistry;2003-03
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