Development of a novel method for specific detection of genetically modified Atlantic salmon, AquAdvantage, using real-time polymerase chain reaction

Author:

Soga Keisuke,Nakamura KosukeORCID,Ishigaki Takumi,Kimata Shinya,Ohmori Kiyomi,Kishine Masahiro,Mano Junichi,Takabatake Reona,Kitta Kazumi,Nagoya Hiroyuki,Kondo Kazunari

Funder

AquaBounty Technologies Inc.

Ministry of Health, Labour and Welfare of Japan

Publisher

Elsevier BV

Subject

General Medicine,Food Science,Analytical Chemistry

Reference26 articles.

1. AquaBounty Technologies, Inc (2010). Environmental Assessment for AquAdvantage® Salmon, An Atlantic salmon (Salmo salar L.) bearing a single copy of the stably integrated α-form of the opAFP-GHc2 gene construct at the α - locus in the EO-1 α line. https://cban.ca/wp-content/uploads/AAS_EA-redacted.pdf. (Last accessed on November 2, 2018).

2. Guidelines for validation of qualitative real-time PCR methods;Broeders;Trends in Food Science & Technology,2014

3. Bundesamt fur Verbraucherschutz und Labensmitelsicherheit (BVL) (2016a). Guidelines for the single-laboratory validation of qualitative real-time PCR methods. https://www.bvl.bund.de/SharedDocs/Downloads/09_Untersuchungen/Guidelines%20for%20the%20single%20laboratory.html (Last accessed on November 2, 2018).

4. Bundesamt fur Verbraucherschutz und Labensmitelsicherheit (BVL) (2016b). Guidelines for the validation of qualitative real-time PCR methods by means of a collaborative study. https://www.bvl.bund.de/SharedDocs/Downloads/09_Untersuchungen/Guidelines%20for%20the%20validation%20of%20qualitative.html. (Last accessed on November 2, 2018).

5. A method to assemble DNA fragments mimicking junctions of transgenic elements: Application to the AquAdvantage salmon;Castro;Food Control,2017

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