Structural characterization of protein–polymer conjugates. I. Assessing heterogeneity of a small PEGylated protein and mapping conjugation sites using ion exchange chromatography and top-down tandem mass spectrometry

Author:

Abzalimov Rinat R.,Frimpong Agya,Kaltashov Igor A.

Publisher

Elsevier BV

Subject

Physical and Theoretical Chemistry,Spectroscopy,Condensed Matter Physics,Instrumentation

Reference30 articles.

1. Effect of PEGylation on pharmaceuticals;Harris;Nat. Rev. Drug Discov.,2003

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3. New PEGs for peptide and protein modification, suitable for identification of the PEGylation site;Veronese;Bioconjug. Chem.,2001

4. Transglutaminase-mediated PEGylation of proteins: direct identification of the sites of protein modification by mass spectrometry using a novel monodisperse PEG;Mero;Bioconjug. Chem.,2009

5. Preparation and characterization of monopegylated human granulocyte-macrophage colony-stimulating factor;Lee;J. Interferon Cytokine Res.,2008

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