Affiliation:
1. College of Horticulture, Fujian Agriculture and Forestry University , Fuzhou 350002 , China
2. Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University , Fuzhou 350002 , China
3. Tea Industry Research Institute, Fujian Agriculture and Forestry University , Fuzhou 350002 , China
Abstract
Abstract
The epitranscriptomic mark N6-methyladenosine (m6A), which is the predominant internal modification in RNA, is important for plant responses to diverse stresses. Multiple environmental stresses caused by the tea-withering process can greatly influence the accumulation of specialized metabolites and the formation of tea flavor. However, the effects of the m6A-mediated regulatory mechanism on flavor-related metabolic pathways in tea leaves remain relatively uncharacterized. We performed an integrated RNA methylome and transcriptome analysis to explore the m6A-mediated regulatory mechanism and its effects on flavonoid and terpenoid metabolism in tea (Camellia sinensis) leaves under solar-withering conditions. Dynamic changes in global m6A level in tea leaves were mainly controlled by two m6A erasers (CsALKBH4A and CsALKBH4B) during solar-withering treatments. Differentially methylated peak-associated genes following solar-withering treatments with different shading rates were assigned to terpenoid biosynthesis and spliceosome pathways. Further analyses indicated that CsALKBH4-driven RNA demethylation can directly affect the accumulation of volatile terpenoids by mediating the stability and abundance of terpenoid biosynthesis-related transcripts and also indirectly influence the flavonoid, catechin, and theaflavin contents by triggering alternative splicing-mediated regulation. Our findings revealed a novel layer of epitranscriptomic gene regulation in tea flavor-related metabolic pathways and established a link between the m6A-mediated regulatory mechanism and the formation of tea flavor under solar-withering conditions.
Funder
Earmarked Fund for China Agriculture Research System of Ministry of Finance and Ministry of Agriculture and Rural Affairs
Scientific Research Foundation of Graduate School of Fujian Agriculture and Forestry University
Scientific Research Foundation of Horticulture College of Fujian Agriculture and Forestry University
Rural Revitalization Tea Industry Technical Service Project of Fujian Agriculture and Forestry University
Fujian Agriculture and Forestry University
6.18 Tea Industry Technology Branch of Collaborative Innovation Institute
Fujian Agriculture and Forestry University Construction Project for Technological Innovation and Service System of Tea Industry Chain
Construction of Plateau Discipline of Fujian Province
Tea Industry Branch of Collaborative Innovation Institute of Fujian Agriculture and Forestry University
Special Fund for Science and Technology Innovation of Fujian Zhang Tianfu Tea Development Foundation
Ministry of Agriculture of the People's Republic of China
Publisher
Oxford University Press (OUP)
Subject
Computational Mathematics,Genetics,Molecular Biology,Biochemistry
Cited by
14 articles.
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