RNA Methylome Reveals the m6A-Mediated Regulation of Flavor Metabolites in Tea Leaves under Solar-Withering

Author:

Zhu Chen123ORCID,Zhang Shuting12ORCID,Zhou Chengzhe123ORCID,Tian Caiyun13ORCID,Shi Biying13ORCID,Xu Kai13ORCID,Huang Linjie13ORCID,Sun Yun13ORCID,Lin Yuling12ORCID,Lai Zhongxiong12ORCID,Guo Yuqiong13ORCID

Affiliation:

1. College of Horticulture, Fujian Agriculture and Forestry University , Fuzhou 350002 , China

2. Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University , Fuzhou 350002 , China

3. Tea Industry Research Institute, Fujian Agriculture and Forestry University , Fuzhou 350002 , China

Abstract

Abstract The epitranscriptomic mark N6-methyladenosine (m6A), which is the predominant internal modification in RNA, is important for plant responses to diverse stresses. Multiple environmental stresses caused by the tea-withering process can greatly influence the accumulation of specialized metabolites and the formation of tea flavor. However, the effects of the m6A-mediated regulatory mechanism on flavor-related metabolic pathways in tea leaves remain relatively uncharacterized. We performed an integrated RNA methylome and transcriptome analysis to explore the m6A-mediated regulatory mechanism and its effects on flavonoid and terpenoid metabolism in tea (Camellia sinensis) leaves under solar-withering conditions. Dynamic changes in global m6A level in tea leaves were mainly controlled by two m6A erasers (CsALKBH4A and CsALKBH4B) during solar-withering treatments. Differentially methylated peak-associated genes following solar-withering treatments with different shading rates were assigned to terpenoid biosynthesis and spliceosome pathways. Further analyses indicated that CsALKBH4-driven RNA demethylation can directly affect the accumulation of volatile terpenoids by mediating the stability and abundance of terpenoid biosynthesis-related transcripts and also indirectly influence the flavonoid, catechin, and theaflavin contents by triggering alternative splicing-mediated regulation. Our findings revealed a novel layer of epitranscriptomic gene regulation in tea flavor-related metabolic pathways and established a link between the m6A-mediated regulatory mechanism and the formation of tea flavor under solar-withering conditions.

Funder

Earmarked Fund for China Agriculture Research System of Ministry of Finance and Ministry of Agriculture and Rural Affairs

Scientific Research Foundation of Graduate School of Fujian Agriculture and Forestry University

Scientific Research Foundation of Horticulture College of Fujian Agriculture and Forestry University

Rural Revitalization Tea Industry Technical Service Project of Fujian Agriculture and Forestry University

Fujian Agriculture and Forestry University

6.18 Tea Industry Technology Branch of Collaborative Innovation Institute

Fujian Agriculture and Forestry University Construction Project for Technological Innovation and Service System of Tea Industry Chain

Construction of Plateau Discipline of Fujian Province

Tea Industry Branch of Collaborative Innovation Institute of Fujian Agriculture and Forestry University

Special Fund for Science and Technology Innovation of Fujian Zhang Tianfu Tea Development Foundation

Ministry of Agriculture of the People's Republic of China

Publisher

Oxford University Press (OUP)

Subject

Computational Mathematics,Genetics,Molecular Biology,Biochemistry

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3