Real time PCR quantification of viable Mycobacterium tuberculosis from sputum samples treated with propidium monoazide

Author:

de Assunção Thiago Milech,Batista Eraldo L.,Deves Candida,Villela Anne Drumond,Pagnussatti Vany ElisaORCID,de Oliveira Dias Ana Christina,Kritski Afrânio,Rodrigues-Junior Valnês,Basso Luiz Augusto,Santos Diógenes Santiago

Funder

Decit/SCTIE/MS-MCT-CNPq-FNDCT-CAPES

FAPERGS-CNPq-PRONEX-2009

CNPq

FAPERGS-CAPES

Publisher

Elsevier BV

Subject

Infectious Diseases,Microbiology (medical),Immunology,Microbiology

Reference35 articles.

1. WHO global tuberculosis report;WHO,2013

2. Clinical diagnosis of smear-negative pulmonary tuberculosis in low-income countries: the current evidence;Siddiqi;Lancet Infect Dis,2003

3. Rapid diagnosis of mycobacterial infections and quantitation of Mycobacterium tuberculosis load by two real-time calibrated PCR assays;Broccolo;J Clin Microbiol,2003

4. Real-time polymerase chain reaction in bronchial aspirate for rapid detection of sputum smear-negative tuberculosis;Min;Int J Tuberc Lung Dis,2010

5. Rapid quantification of viable Campylobacter bacteria on chicken carcasses, using real-time PCR and propidium monoazide treatment, as a tool for quantitative risk assessment;Josefsen;Appl Environ Microbiol,2010

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