PCR procedures to amplify GC-rich DNA sequences of Mycobacterium bovis
Author:
Funder
Canadian Food Inspection Agency
Publisher
Elsevier BV
Subject
Microbiology (medical),Molecular Biology,Microbiology
Reference13 articles.
1. Primer designing for PCR;Borah;Sci. Vis.,2011
2. “Touchdown” PCR to circumvent spurious priming during gene amplification;Don;Nucleic Acids Res.,1991
3. Two alternative DNA extraction methods to improve the detection of Mycobacterium-tuberculosis-complex members in cattle and red deer tissue samples;Fell;BMC Microbiol.,2016
4. PCR-amplification of GC-rich regions: “slowdown PCR”;Frey;Nat. Protoc.,2008
5. DMSO and betaine greatly improve amplification of GC- rich constructs in De novo synthesis;Jensen;PLoS One,2010
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