PCR-amplification of GC-rich regions: 'slowdown PCR'-Reference-Cited by-同舟云学术

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PCR-amplification of GC-rich regions: 'slowdown PCR'

Published:2008-07-24 Issue:8 Volume:3 Page:1312-1317
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Frey Ulrich H,Bachmann Hagen S,Peters Jürgen,Siffert Winfried

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://www.nature.com/articles/nprot.2008.112.pdf

Reference35 articles.

1. Mullis, K.B. & Faloona, F.A. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 155, 335–350 (1987).

2. Saiki, R.K. et al. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487–491 (1988).

3. Roux, K.H. Optimization and troubleshooting in PCR. PCR Methods Appl. 4, S185–S194 (1995).

4. McDowell, D.G., Burns, N.A. & Parkes, H.C. Localised sequence regions possessing high melting temperatures prevent the amplification of a DNA mimic in competitive PCR. Nucleic Acids Res. 26, 3340–3347 (1998).

5. Varadaraj, K. & Skinner, D.M. Denaturants or cosolvents improve the specificity of PCR amplification of a G + C-rich DNA using genetically engineered DNA polymerases. Gene 140, 1–5 (1994).

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