RNA-seq data exploration after trypanosome RNA-binding protein UBP1 expression is altered by CRISPR-Cas9 gene editing and overexpression

Author:

Sabalette Karina B.,Campo Vanina A.,Sotelo-Silveira José R.,Smircich Pablo,De Gaudenzi Javier G.

Funder

ICGEB

Publisher

Elsevier BV

Subject

Multidisciplinary

Reference6 articles.

1. Insights into the Regulation of mRNA Processing of Polycistronic Transcripts Mediated by DRBD4/PTB2, a Trypanosome Homolog of the Polypyrimidine Tract-Binding Protein;De Gaudenzi;J EukaryotMicrobiol,2016

2. A 43-nucleotide U-rich element in 3′-untranslated region of large number of Trypanosoma cruzi transcripts is important for mRNA abundance in intracellular amastigotes;Li;J Biol Chem,2012

3. RNA-Seq reveals that overexpression of TcUBP1 switches the gene expression pattern toward that of the infective form of Trypanosoma cruzi;Sabalette;J BiolChem,2023

4. Sabalette KB, Campo VA, Sotelo-Silveira JR, Smircich P, De Gaudenzi JG. Transcriptomic analysis of N-terminal mutated Trypanosoma cruzi UBP1 knockdown underlines the importance of this RNA-binding protein in parasite development. bioRxiv 2023.12.07.570581; 10.1101/2023.12.07.570581

5. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2;Love;Genome Biol,2014

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