A multifunctional vector system for heterologous expression of proteins in Escherichia coli Expression of native and hexahistidyl fusion proteins, rapid purification of the fusion proteins, and removal of fusion peptide by Kex2 protease-Reference-Cited by-同舟云学术

A multifunctional vector system for heterologous expression of proteins in Escherichia coli Expression of native and hexahistidyl fusion proteins, rapid purification of the fusion proteins, and removal of fusion peptide by Kex2 protease

Published:1996-10 Issue:1-2 Volume:176 Page:249-255
ISSN:0378-1119
Container-title:Gene
language:en
Short-container-title:Gene

Author:

Ghosh Smita,Lowenstein John M.

Publisher

Elsevier BV

Subject

Genetics,General Medicine

Reference20 articles.

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4. Eukaryotic proteins expressed in E. coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase;Guan;Anal. Biochem.,1991

5. Expression of bovine growth hormone derivatives in Escherichia coli and the use of derivatives to produce natural sequence growth hormone by cathepsin C cleavage;Hsiung;Meth. Enzymol.,1987

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