Expression of chymotrypsin(ogen) in the thioredoxin reductase deficient mutant strain of Escherichia coli AD494(DE3) and purification via a fusion product with a hexahistidine-tail-Reference-Cited by-同舟云学术

Expression of chymotrypsin(ogen) in the thioredoxin reductase deficient mutant strain of Escherichia coli AD494(DE3) and purification via a fusion product with a hexahistidine-tail

Published:2000-01 Issue:1-2 Volume:737 Page:213-224
ISSN:0378-4347
Container-title:Journal of Chromatography B: Biomedical Sciences and Applications
language:en
Short-container-title:Journal of Chromatography B: Biomedical Sciences and Applications

Author:

Verheyden Gert,Volckaert Guido,Engelborghs Yves

Publisher

Elsevier BV

Subject

General Chemistry

Reference44 articles.

1. Modelling pathways of alpha-chymotrypsin activation and deactivation

2. Structure of crystalline α-chymotrypsin

3. Chymotrypsinogen: 2,5-Å crystal structure, comparison with α-chymotrypsin, and implications for zymogen activation

4. Expression of rat chymotrypsinogen in yeast: a study on the structural and functional significance of the chymotrypsinogen propeptide

5. Arginine 127 stabilizes the transition state in carboxypeptidase

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1. Overexpression of a bacterial chymotrypsin: Application for l-amino acid ester hydrolysis;Enzyme and Microbial Technology;2011-12

2. Simulation of the activation of α-chymotrypsin: Analysis of the pathway and role of the propeptide;Protein Science;2009-01-01

3. Exploration of the activation pathway of Δα-Chymotrypsin with molecular dynamics simulations and correlation with kinetic experiments;European Biophysics Journal;2008-08-27

4. Molecular and structural characterization of a trypsin highly expressed in larval stage ofZabrotes subfasciatus;Archives of Insect Biochemistry and Physiology;2007

5. A fluorescence stopped-flow kinetic study of the conformational activation of α-chymotrypsin and several mutants;Protein Science;2004-09