Phosphorothioate-based ligase-independent gene cloning (PLICing): An enzyme-free and sequence-independent cloning method

Author:

Blanusa Milan,Schenk Alexander,Sadeghi Hengameh,Marienhagen Jan,Schwaneberg Ulrich

Funder

Jacobs University

Publisher

Elsevier BV

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

Reference18 articles.

1. Molecular Cloning: A Laboratory Manual;Maniatis,1982

2. Ligation-independent cloning of PCR products (LIC–PCR);Aslanidis;Nucleic Acids Res.,1990

3. Universal cloning method by TA strategy;Zhou;Biotechniques,1995

4. Enzyme-free cloning: a rapid method to clone PCR products independent of vector restriction enzyme sites;Tillett;Nucleic Acids Res.,1999

5. Minimal length requirement of the single-stranded tails for ligation-independent cloning (LIC) of PCR products;Aslanidis;PCR Methods Appl.,1994

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