Development of PCR primers enabling the design of flexible sticky ends for efficient concatenation of long DNA fragments-Reference-Cited by-同舟云学术

Development of PCR primers enabling the design of flexible sticky ends for efficient concatenation of long DNA fragments

Published:2024 Issue:4 Volume:5 Page:360-371
ISSN:2633-0679
Container-title:RSC Chemical Biology
language:en
Short-container-title:RSC Chem. Biol.

Author:

Nomura Kohei¹,Onda Kaoru¹,Murase Hirotaka¹,Hashiya Fumitaka²³,Ono Yukiteru⁴,Terai Goro⁴,Oka Natsuhisa⁵^ORCID,Asai Kiyoshi⁴,Suzuki Daisuke¹,Takahashi Naho¹,Hiraoka Haruka¹^ORCID,Inagaki Masahito¹,Kimura Yasuaki¹^ORCID,Shimizu Yoshihiro⁶,Abe Naoko¹,Abe Hiroshi¹²³⁷^ORCID

Affiliation:

1. Department of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan

2. Research Center for Materials Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan

3. CREST, Japan Science and Technology Agency, 7 Gobancho, Chiyoda-ku, Tokyo 102-0076, Japan

4. Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwanoha, Kashiwa, Chiba 277-8561, Japan

5. Department of Chemistry and Biomolecular Science Faculty of Engineering, Gifu University, Gifu 501-1193, Japan

6. Laboratory for Cell-Free Protein Synthesis, RIKEN Center for Biosystems Dynamics Research, Suita, Osaka 565-0874, Japan

7. Institute for Glyco-core Research (iGCORE), Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan

Abstract

We report on chemically modified PCR primers that enable the design of flexible sticky ends by introducing a photo-cleavable group at the phosphate moiety. DNA fragments synthesized using these primers can be ligated with higher efficiency.

Funder

Core Research for Evolutional Science and Technology

Japan Agency for Medical Research and Development

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/CB/D3CB00212H

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