Endogenous RNase inhibitor contributes to stability of RNA in crude cell lysates: Applicability to RT-qPCR-Reference-Cited by-同舟云学术

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Endogenous RNase inhibitor contributes to stability of RNA in crude cell lysates: Applicability to RT-qPCR

Published:2016-11 Issue: Volume:513 Page:21-27
ISSN:0003-2697
Container-title:Analytical Biochemistry
language:en
Short-container-title:Analytical Biochemistry

Author:

Wang Xiao,Teferedegne Belete,Shatzkes Kenneth,Tu Wei,Murata Haruhiko

Funder

U.S. Food and Drug Administration

Publisher

Elsevier BV

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

Reference17 articles.

1. Accurate RT-qPCR gene expression analysis on cell culture lysates;Van Peer;Sci. Rep.,2012

2. Direct quantification of mRNA and miRNA from cell lysates using reverse transcription real time PCR: a multidimensional analysis of the performance of reagents and workflows;Ho;PLoS One,2013

3. A simple, inexpensive method for preparing cell lysates suitable for downstream reverse transcription quantitative PCR;Shatzkes;Sci. Rep.,2014

4. Development of a neutralization assay for influenza virus using an endpoint assessment based on quantitative reverse-transcription PCR;Teferedegne;PLoS One,2013

5. A neutralization assay for respiratory syncytial virus using a quantitative PCR-based endpoint assessment;Varada;Virol. J.,2013

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