A simple, inexpensive method for preparing cell lysates suitable for downstream reverse transcription quantitative PCR
Author:
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Link
http://www.nature.com/articles/srep04659.pdf
Reference17 articles.
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2. Varada, J. C. et al. A neutralization assay for respiratory syncytial virus using a quantitative PCR-based endpoint assessment. Virology J 10, 195 (2013).
3. Abruzzese, R. V., Fekete, R. & Kephart, D. Cells-to-CT kits: Next generation gene expression analysis workflows that eliminate sample purification. J Lab Autom 15, 362–368 (2010).
4. Gong, E. Y. et al. A duplex real-time RT-PCR assay for profiling inhibitors of four dengue serotypes. Methods Mol Biol 1030, 195–203 (2013).
5. Van Peer, G., Mestdagh, P. & Vandesompele, J. Accurate RT-qPCR gene expression analysis on cell culture lysates. Sci Rep 2, 222 (2012).
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