Identification of a T7 RNA polymerase variant that permits the enzymatic synthesis of fully 2′-O-methyl-modified RNA-Reference-Cited by-同舟云学术

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Identification of a T7 RNA polymerase variant that permits the enzymatic synthesis of fully 2′-O-methyl-modified RNA

Published:2013-09 Issue:3 Volume:167 Page:287-295
ISSN:0168-1656
Container-title:Journal of Biotechnology
language:en
Short-container-title:Journal of Biotechnology

Author:

Ibach Jenny,Dietrich Laura,Koopmans Kyra R.M.,Nöbel Nico,Skoupi Marc,Brakmann Susanne

Publisher

Elsevier BV

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology,Bioengineering

Reference37 articles.

1. Fully 2′-modified oligonucleotide duplexes with improved in vitro potency and stability compared to unmodified small interfering RNA;Allerson;Journal of Medicinal Chemistry,2005

2. Bleach gel: a simple agarose gel for analyzing RNA quality;Aranda;Electrophoresis,2012

3. An error-prone T7 RNA polymerase mutant generated by directed evolution;Brakmann;ChemBioChem,2001

4. 2-Deoxy purine, 2-O-methyl pyrimidine (dRmY) aptamers as candidate therapeutics;Burmeister;Oligonucleotides,2006

5. Direct in vitro selection of a 2′-O-methyl aptamer to VEGF;Burmeister;Chemistry & Biology,2005

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