Effect of storage time on peripheral blood mononuclear cell isolation from blood collected in vacutainer CPT™ tubes

Author:

Linggi Bryan,Cremer Jonathan,Wang Zhongya,Van Viegen Tanja,Vermeire Séverine,Lefevre Pavine,Shackelton Lisa M.,Jairath Vipul,Teft Wendy,Vande Casteele Niels,Verstockt Bram

Publisher

Elsevier BV

Subject

Immunology,Immunology and Allergy

Reference21 articles.

1. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1g;Boyum;Scand. J. Clin. Lab. Investig. Suppl.,1968

2. Defining blood processing parameters for optimal detection of cryopreserved antigen-specific responses for HIV vaccine trials;Bull;J. Immunol. Methods,2007

3. Immune cell subsets and their gene expression profiles from human PBMC isolated by vacutainer cell preparation tube (CPT) and standard density gradient;Corkum;BMC Immunol.,2015

4. Changes in expression of PD-L1 on peripheral T cells in patients with melanoma and lung cancer treated with PD-1 inhibitors;Dart;Sci. Rep.,2021

5. Analysis of complex biomarkers for human immune-mediated disorders based on cytokine responsiveness of peripheral blood cells;Davis;J. Immunol.,2010

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