Optimization of Peripheral Blood Mononuclear Cell Processing for Improved Clinical ELISpot Assay Performance
Author:
Publisher
Springer Science and Business Media LLC
Subject
Pharmaceutical Science
Link
https://link.springer.com/content/pdf/10.1208/s12248-023-00861-y.pdf
Reference24 articles.
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3. Afonso G, Scotto M, Renand A, Arvastsson J, Vassilieff D, Cilio CM, et al. Critical parameters in blood processing for T-cell assays: validation on ELISpot and tetramer platforms. J Immunol Methods. 2010;359(1–2):28–36.
4. Bull M, Lee D, Stucky J, Chiu YL, Rubin A, Horton H, et al. Defining blood processing parameters for optimal detection of cryopreserved antigen-specific responses for HIV vaccine trials. J Immunol Methods. 2007;322(1–2):57–69.
5. Kierstead LS, Dubey S, Meyer B, Tobery TW, Mogg R, Fernandez VR, et al. Enhanced rates and magnitude of immune responses detected against an HIV vaccine: effect of using an optimized process for isolating PBMC. AIDS Res Hum Retroviruses. 2007;23(1):86–92.
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