Bacterial chemotactic motility is important for the initiation of wheat root colonization by Azospirillum brasilense

Abstract

Bacteria of the genusAzospirillumare able to colonize plant roots. Using the glucuronidase (GUS) reporter system, variousAzospirillummutants, including mutants affected in chemotactic motility or extracellular polysaccharide biosynthesis, were investigated for their capacity to initiate wheat root colonization at the root hair zones. Only non-flagellated mutants and a generally non-chemotactic mutant exhibited a strongly reduced colonization ability as compared to the wild-type. No role of theAzospirillumcalcofluor-binding polysaccharide in primary wheat root colonization could be observed. This is the first report demonstrating directly, by using different motility mutants, the requirement of bacterial motility in the establishment of theAzospirillum-plant root association.