Cloning and characterization of the ColE7 plasmid-Reference-Cited by-同舟云学术

Cloning and characterization of the ColE7 plasmid

Published:1991-01-01 Issue:1 Volume:137 Page:91-100
ISSN:1350-0872
Container-title:Microbiology
language:en
Short-container-title:

Author:

Chak Kin-F.¹,Kuo White-S.¹,Lu fong-m¹,James R.²

Affiliation:

1. Department of Biochemistry, National Yang-Ming Medical College, Shih-Pai, Taipei, Taiwan 112, ROC

2. School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK

Abstract

Summary: The 2·6 kb ColE7-K317 plasmid was mapped and the DNA fragments of the colicin E7 operon subcloned into pUC18 and pUC19. The size of the functional colicin E7 operon deduced by subcloning was 2·3 kb. The colicin E7 gene product was purified by carboxymethylcellulose chromatography. Both colicin E7 and E9 were demonstrated to exhibit a non-specific DNAase-type activity by in vitro biological assay. The molecular mass of colicin E7 was 61 kDa, as determined by SDS-PAGE. From DNA sequence data, the estimated sizes of the E7 immunity protein and the E7 lysis protein were 9926 Da and 4847 Da, respectively. Comparison of restriction maps and DNA sequence data suggests that ColE7 and ColE2 are more closely related than other E colicin plasmids.

Publisher

Microbiology Society

Subject

Microbiology

Reference32 articles.

1. Specific inactivation of 16S ribosomal RNA induced by colicin E3 in vivo;Bowman,1971

2. A rapid alkaline extraction procedure for screening plasmid DNA;Birnboim;Methods in Enzymology,1983

3. Localization and characterization of a gene on the ColE3-CA38 plasmid that confers immunity to colicin E8;Chak;Journal of General Microbiology,1984

4. Analysis of the promoters for the two immunity genes present in the ColE3-CA38 plasmid using two new promoter probe vectors;Chak;Nucleic Acids Research,1985

5. Characterization of the ColE9-J plasmid and analysis of its genetic organization;Chak;Journal of General Microbiology,1986

Cited by 91 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. The dynamics of colicin E9 release from Escherichia coli in native conditions;Letters in Applied Microbiology;2024-04-23

2. Identification and characterization of a new HNH restriction endonuclease with unusual properties;Applied Microbiology and Biotechnology;2023-08-26

3. Hydrolytic Mechanism of a Metalloenzyme Is Modified by the Nature of the Coordinated Metal Ion;Molecules;2023-07-19

4. Intercepting biological messages: Antibacterial molecules targeting nucleic acids during interbacterial conflicts;Genetics and Molecular Biology;2023

5. A DNase Type VI Secretion System Effector Requires Its MIX Domain for Secretion;Microbiology Spectrum;2022-10-26