Inactivation of avian influenza viruses by hydrostatic pressure as a potential vaccine development approach

Author:

Barroso Shana Priscila Coutinho123ORCID,Vicente dos Santos Ana Clara12,Souza dos Santos Patrícia412,dos Santos Silva Couceiro José Nelson5,Fernandes Ferreira Davis5ORCID,Nico Dirlei5ORCID,Morrot Alexandre67ORCID,Lima Silva Jerson21ORCID,Cheble de Oliveira Andrea12

Affiliation:

1. Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem, Brazil

2. Laboratório de Termodinâmica de Proteínas e Estruturas Virais Gregorio Weber, Programa de Biologia Estrutural, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, 21941-590, Rio de Janeiro, RJ, Brazil

3. Laboratório de Biologia Molecular, Instituto de Pesquisas Biomédicas, Hospital Naval Marcílio Dias, Marinha do Brasil, Brazil

4. Centro Universitário IBMR, Rio de Janeiro, RJ, Brazil

5. Departamento de Virologia, Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

6. Faculdade de Medicina, Departamento de Clínica Médica, Centro de Pesquisa em Tuberculose,, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

7. Laboratório de Imunoparasitologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brazil

Abstract

Vaccines are a recommended strategy for controlling influenza A infections in humans and animals. Here, we describe the effects of hydrostatic pressure on the structure, morphology and functional characteristics of avian influenza A H3N8 virus. The effect of hydrostatic pressure for 3 h on H3N8 virus revealed that the particles were resistant to this condition, and the virus displayed only a discrete conformational change. We found that pressure of 3 kbar applied for 6 h was able to inhibit haemagglutination and infectivity while virus replication was no longer observed, suggesting that full virus inactivation occurred at this point. However, the neuraminidase activity was not affected at this approach suggesting the maintenance of neutralizing antibody epitopes in this key antigen. Our data bring important information for the area of structural virology of enveloped particles and support the idea of applying pressure-induced inactivation as a tool for vaccine production.

Funder

CNPq

CAPES

FAPERJ

IMBEBB

INBEB

PRONEX

Publisher

Microbiology Society

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