Case report: a genomics-guided reclassification of a blood culture isolate misassigned by MALDI-TOF as Yersinia pestis-Reference-Cited by-同舟云学术

Case report: a genomics-guided reclassification of a blood culture isolate misassigned by MALDI-TOF as Yersinia pestis

Published:2022-10-03 Issue:10 Volume:4 Page:
ISSN:2516-8290
Container-title:Access Microbiology
language:en
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Author:

Sim Eby M.¹^ORCID,Koh Bryant¹,Ng Jimmy¹,Nguyen Trang¹,Wang Qinning¹,Ginn Andrew N.²¹,Brown Mitchell¹,Pham David¹,Sintchenko Vitali³²¹

Affiliation:

1. Centre for Infectious Diseases and Microbiology- Laboratory Services, Institute of Clinical Pathology and Medical Research, NSW Health Pathology, Sydney, New South Wales, Australia

2. Sydney Institute for Infectious Diseases, The University of Sydney, Sydney, New South Wales, Australia

3. Centre for Infectious Diseases and Microbiology- Public Health, Westmead Hospital, Sydney, New South Wales, Australia

Abstract

In this report, we describe a case where Gram-negative rods were isolated from a blood culture which subsequently presented a discordant

Yersinia

species result by MALDI-TOF. Rapid sequencing provided high-resolution identification of the isolate as

Yersinia pseudotuberculosis

, which was subsequently confirmed by biochemical tests.

Publisher

Microbiology Society

Subject

General Medicine

Reference22 articles.

1. Use of Whole-Genus Genome Sequence Data To Develop a Multilocus Sequence Typing Tool That Accurately Identifies Yersinia Isolates to the Species and Subspecies Levels

2. Early Divergent Strains of Yersinia pestis in Eurasia 5,000 Years Ago

3. Insights into the evolution of Yersinia pestis through whole-genome comparison with Yersinia pseudotuberculosis

4. Misidentification of Yersinia pestis by Automated Systems, Resulting in Delayed Diagnoses of Human Plague Infections--Oregon and New Mexico, 2010-2011

5. Yersinia pseudotuberculosis bacteraemia: a diagnostic dilemma in the era of MALDI-TOF mass spectrometry

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