Characterization of meticillin-resistant Staphylococcus aureus isolates from hospitals in KwaZulu-Natal province, Republic of South Africa

Author:

Shittu Adebayo12,Nübel Ulrich3,Udo Edet4,Lin Johnson1,Gaogakwe Sedio1

Affiliation:

1. School of Biochemistry, Genetics and Microbiology, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban, Republic of South Africa

2. Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Nigeria

3. Robert Koch Institute, 38855 Wernigerode, Germany

4. Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait

Abstract

Epidemiological data based on phenotypic and molecular characterization of meticillin-resistantStaphylococcus aureus(MRSA) in sub-Saharan Africa are limited. This investigation studied 61 MRSA isolates obtained from 13 health-care institutions in KwaZulu-Natal (KZN) province, South Africa, from March 2001 to August 2003. More than 80 % of the isolates were resistant to at least four classes of antibiotics and six isolates were resistant to the aminoglycoside, macrolide-lincosamide and tetracycline groups of antibiotics, heavy metals and nucleic acid-binding compounds. PFGE ofSmaI-digested genomic DNA revealed seven types, designated A–G. Type A was the main pulsotype (62.3 %) and was identified in 11 of the 13 health-care institutions, suggesting that it represented a major clone in health-care institutions in KZN province. Analysis of representative members of the three major pulsotypes byspa, multilocus sequence typing and SCCmectyping revealed the types t064-ST1173-SCCmecIV and t064-ST1338-SCCmecIV (PFGE type A, single-locus and double-locus variants of ST8), t037-ST239-SCCmecIII (PFGE type F) and t045-ST5-SCCmecIII (PFGE type G). The combination of various typing methods provided useful information on the geographical dissemination of MRSA clones in health-care institutions in KZN province. The observation of major clones circulating in health-care facilities in KZN province indicates that adequate infection control measures are urgently needed.

Publisher

Microbiology Society

Subject

Microbiology (medical),General Medicine,Microbiology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3