Isolation, Identification, and Antimicrobial Profiling of Bacteria from Aquaculture Fishes in Pond Water of Bangladesh

Abstract

Bacterial diseases are widespread and can be of particular importance in the fish farming of Bangladesh. This investigation was done to assess and compare the bacteria diversities and population in local fresh water pond fishes. Out of 95 samples, 54(56.9%) were Shing (Heteropneustes fossils), 14(14.7%) were Pangas (Pangasius pangasius), 9(9.5%) were Pabda (Ompok spp), 7(7.3%) were Thai Koi, (Anabas testudineus), and 11(11.6%) were others infected fishes such as Shol (Channa striata), Magur (Clarias spp), Tilapia (Oreochromis niloticus), and Tengara (Mystus cavasius) fishes. Among 95 infected fishes 84(88.4%) were infected with pathogenic bacteria and 11(11.6%) were normal flora. There were eight types of different isolated spp with the frequency of occurrence were 36 (42.9%) Aeromonas spp, 15 (17.9%) Pseudomonas spp, 7(8.3%) Vibrio spp, 9 (10.7%) Staphylococcus spp, 7(8.3%) Flavobacterium spp, 7(8.3%) Edwardsiella spp, others were 3(3.6%) Citobacter spp, and Enterobacter spp respectively. All of the isolated pathogenic bacteria showed 84/84(100%) resistant to Amoxicillin and 18/84(64.3%) resistant to Erythromycin. All the strains showed sensitive to Ciprofloxacin, Cotrimoxazole, Enorfloxacin, Doxyciline, Clotetracycline, and Colistin with the frequency of occurrence were 78/84(92.8%), 76/84 (90.5%), 73/84(86.9%), 67/84(67.9%), 53/84(63.1%) and 52/84(61.9%) respectively. The physiochemical characteristics of 95 pond water samples were analyzed; the average water temperature, pH, and ammonia were 27.3oC, 7.6, and 0.87mg/L respectively. The significant variation in the physiochemical parameters like water temperature, pH, ammonia was observed within these five types of ponds water. Hence, it is important to detect fish diseases, responsible pathogens, and other agents for the protection of our water resources.