Luteolibacter luojiensis sp. nov., isolated from Arctic tundra soil, and emended description of the genus Luteolibacter

Abstract

A yellow-pigmented, Gram-reaction-negative, non-motile, aerobic bacterium, designated DR4-30T, was isolated from tundra soil near Ny-Ålesund, Svalbard Archipelago, Norway (78° 58′ N 12° 03′ E). Growth occurred at 4–28 °C (optimum 20–25 °C) and at pH 7–8 (optimum pH 7). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain DR4-30T belongs to the genus Luteolibacter in the family Verrucomicrobiaceae . The 16S rRNA gene sequence of this strain showed 95.4 and 94.7 % sequence similarity to those of Luteolibacter pohnpeiensis A4T-83T and Luteolibacter algae A5J-41-2T, respectively. The major respiratory quinones were MK-9 and MK-10; the predominant cellular fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 20.7 %), iso-C14 : 0 (20.3 %), C17 : 0 (10.7 %), C16 : 0 (8.0 %) and C14 : 0 (6.6 %). The DNA G+C content was 57.3 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain DR4-30T represents a novel species of the genus Luteolibacter , for which the name Luteolibacter luojiensis sp. nov. is proposed. The type strain is DR4-30T ( = CCTCC AB 2010415T  = NRRL B-59669T). An emended description of the genus Luteolibacter is also provided.