Affiliation:
1. Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, 315800, PR China
2. Ningbo Institute of Marine Medicine, Peking University, Ningbo, 315800, PR China
Abstract
A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium, designated NBU1238T, was isolated from marine sediment sampled on Meishan Island located in the East China Sea. Strain NBU1238T was able to grow optimally at 28–32 °C, at pH 7.5 and with no NaCl. Catalase and oxidase activities, H2S production and hydrolysis of Tweens 40 and 60 were positive. Methyl red reaction, Voges–Proskauer test and hydrolysis of starch, casein and Tweens 20 and 80 were negative. The major cellular fatty acids were iso-C14 : 0, C16 : 0, C16 : 1
ω9c and C14 : 0. The only respiratory quinone was menaquinone-9. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylmethylethanolamine. The 16S rRNA gene sequence of strain NBU1238T showed 95.6, 95.6, 94.8 and 93.8% sequence similarity to
Luteolibacter flavescens
GKXT,
Luteolibacter luteus
G-1-1-1T,
Luteolibacter arcticus
MC 3726T and
Luteolibacter pohnpeiensis
A4T-83T, respectively. Phylogenetic analyses indicated that strain NBU1238T clustered with the genus
Luteolibacter
and was closely related to strains
L. flavescens
GKXT,
L. arcticus
MC 3726T and
L. luteus
G-1-1-1T. The average nucleotide identity and digital DNA–DNA hybridization values between strain NBU1238T and related species of genus
Luteolibacter
were well below the threshold limit for prokaryotic species delineation. The DNA G+C content of the genomic DNA was 65.0 mol%. Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU1238T is considered to be a representative of a novel species in the genus
Luteolibacter
, for which the name Luteolibacter marinus sp. nov. is proposed. The type strain is NBU1238T (=KCTC 82227T=MCCC 1K04772T).
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
1 articles.
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