Pseudomonas chengduensis sp. nov., isolated from landfill leachate

Abstract

Strain MBRT was isolated from landfill leachate in a solid-waste disposal site in Chengdu, Sichuan, China. An analysis of 16S rRNA gene sequences revealed that the isolate was closely related to members of the genus Pseudomonas , sharing the highest sequence similarities with Pseudomonas toyotomiensis HT-3T (99.8 %), Pseudomonas alcaliphila AL15-21T (99.7 %) and Pseudomonas oleovorans ATCC 8062T (99.4 %). Multi-locus sequence analysis based on three housekeeping genes (gyrB, rpoB and rpoD) provided higher resolution at the species level than that based on 16S rRNA gene sequences, which was further confirmed by less than 70 % DNA–DNA relatedness between the new isolate and P. toyotomiensis HT-3T (61.3 %), P. alcaliphila AL15-21T (51.5 %) and P. oleovorans ATCC 8062T (57.8 %). The DNA G+C content of strain MBRT was 61.9 mol% and the major ubiquinone was Q-9. The major cellular fatty acids (>10 %) were C18 : 1ω7c and/or C18 : 1ω6c, C16 : 0, and C16 : 1ω7c and/or C16 : 1ω6c. Polyphasic analysis indicates that strain MBRT represents a novel species of the genus Pseudomonas , for which the name Pseudomonas chengduensis sp. nov. is proposed. The type strain is MBRT ( = CGMCC 2318T = DSM 26382T).