Affiliation:
1. Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences & Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, PR China
2. College of Life Science, Sichuan University, Chengdu 610064, PR China
Abstract
Strain MBRT was isolated from landfill leachate in a solid-waste disposal site in Chengdu, Sichuan, China. An analysis of 16S rRNA gene sequences revealed that the isolate was closely related to members of the genus
Pseudomonas
, sharing the highest sequence similarities with
Pseudomonas toyotomiensis
HT-3T (99.8 %),
Pseudomonas alcaliphila
AL15-21T (99.7 %) and
Pseudomonas oleovorans
ATCC 8062T (99.4 %). Multi-locus sequence analysis based on three housekeeping genes (gyrB, rpoB and rpoD) provided higher resolution at the species level than that based on 16S rRNA gene sequences, which was further confirmed by less than 70 % DNA–DNA relatedness between the new isolate and
P. toyotomiensis
HT-3T (61.3 %),
P. alcaliphila
AL15-21T (51.5 %) and
P. oleovorans
ATCC 8062T (57.8 %). The DNA G+C content of strain MBRT was 61.9 mol% and the major ubiquinone was Q-9. The major cellular fatty acids (>10 %) were C18 : 1ω7c and/or C18 : 1ω6c, C16 : 0, and C16 : 1ω7c and/or C16 : 1ω6c. Polyphasic analysis indicates that strain MBRT represents a novel species of the genus
Pseudomonas
, for which the name Pseudomonas chengduensis sp. nov. is proposed. The type strain is MBRT ( = CGMCC 2318T = DSM 26382T).
Funder
West Light Foundation of the Chinese Academy of Sciences
National Natural Science Foundation of China
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
35 articles.
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