Affiliation:
1. Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences, Key Laboratory of Plant Nutrition and Fertilizer in South Region, Ministry of Agriculture, Key Laboratory of Nutrient Cycling and Farmland Conservation of Guangdong Province, Guangzhou 510640, China
2. Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming 525000, China
3. Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
4. Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China
Abstract
Pseudomonas chengduensis is a new species of Pseudomonas discovered in 2014, and currently, there is a scarcity of research on this bacterium. The P. chengduensis strain WD211 was isolated from a fish pond. This study investigated the purification capability and environmental adaptability of strain WD211 in wastewater and described the basic features and functional genes of its complete genome. According to the results, the sewage treated with strain WD211 showed a decrease in concentration of 18.12% in total nitrogen, 89.39% in NH4+, 62.16% in NO3−, 79.97% in total phosphorus, and 71.41% in COD after 24 h. Strain WD211 is able to survive in a pH range of 6–11. It shows resistance to 7% sodium chloride and different types of antibiotics. Genomic analysis showed that strain WD211 may remove nitrogen and phosphorus through the metabolic pathway of nitrogen assimilation and phosphorus accumulation, and that it can promote organic decomposition through oxygenase. Strain WD211 possesses genes for producing betaine, trehalose, and sodium ion transport, which provide it with salt tolerance. It also has genes for antibiotic efflux and multiple oxidases, which give it antibiotic resistance. This study contributes to the understanding of the sewage treatment ability and potential applications of P. chengduensis.
Funder
Fisheries Research Center Project of Guangdong Academy of Agriculture Science
Science and Technology Program of Guangzhou, China
Guangdong Academy of Agricultural Sciences
Rural Revitalization Strategy of Guangdong
The open competition program of top nine critical priorities of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province
Guangdong Basic and Applied Basic Research Foundation
Agricultural competitive industry discipline team building project of Guangdong Academy of Agricultural Sciences
Start-up Research Project of Maoming Laboratory
Guangdong Province Pig Cross-County Cluster Industrial Park
High-level Foreign Expert Project of Guangdong Province
Science and technology cooperation special project of Guangdong Academy of Agricultural Sciences, Shanwei Branch
Subject
Genetics (clinical),Genetics