Affiliation:
1. State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China
2. School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China
Abstract
A taxonomic study was carried out on strain GCS-AN-3T, which was isolated from a phenol-degrading consortium enriched from coking wastewater activated sludge of Beijing Shougang Company Limited during the screening of phenol-degrading bacteria. Cells of strain GCS-AN-3T were Gram-stain-negative, short rods, and oxidase-/catalase-positive. Growth was observed at salinities from 0 to 2.5 % and at temperatures from 10 to 37 °C. 16S rRNA gene sequence analysis showed that strain GCS-AN-3T was most closely related to
Ottowia pentelensis
DSM 21699T (96.2 %). The principal fatty acids were summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), C16 : 0, summed feature 8 (C18 : 1ω7c/C18 : 1ω6c) and cyclo C17 : 0. The major respiratory quinone was Q-8. The polar lipids comprised phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and an unknown phospholipid. The G+C content of the genomic DNA was 67.6 mol%. Thiosulfate could be utilized as co-substrate for aerobic growth and was oxidized to sulfate. On the basis of phenotypic, chemotaxonomic and molecular data, strain GCS-AN-3T is considered to represent a novel species of the genus
Ottowia
, for which the name Ottowia beijingensis sp. nov. is proposed (type strain GCS-AN-3T = LMG 27179T = CGMCC 1.12324T = MCCC 1A01410T). An emended description of the genus
Ottowia
is also proposed.
Funder
National Infrastructure of Natural Resources for Science and Technology Program of China
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Reference22 articles.
1. [Isolation of phenol-degrading bacteria from coking wastewater and their degradation gene];Cao;Huan Jing Ke Xue,2011
2. Isoprenoid quinone analysis in classification and identification;Collins,1985
3. Ottowia pentelensis sp. nov., a floc-forming betaproteobacterium isolated from an activated sludge system treating coke plant effluent;Felföldi;Int J Syst Evol Microbiol,2011
4. Evolutionary trees from DNA sequences: a maximum likelihood approach;Felsenstein;J Mol Evol,1981
Cited by
25 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献