Ottowia pentelensis sp. nov., a floc-forming betaproteobacterium isolated from an activated sludge system treating coke plant effluent

Abstract

A Gram-negative-staining, short-rod-shaped, floc-forming bacterium, designated strain RB3-7T, was isolated from a laboratory-scale activated sludge system treating coke plant effluent. Comparative analysis of the 16S rRNA gene sequence demonstrated that the novel isolate was distantly related (≤95.8 % similarity) toOttowia thiooxydansK11Twithin the familyComamonadaceae. Strain RB3-7Twas catalase- and oxidase-positive and non-motile. The predominant fatty acids were C16 : 0, cyclo C17 : 0, C18 : 1ω7cand C16 : 1ω7c, and the major respiratory quinone was Q-8. The G+C content of the genomic DNA of strain RB3-7Twas 68.5 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain RB3-7Tis considered to represent a novel species of the genusOttowia, for which the nameOttowia pentelensissp. nov. is proposed. The type strain is RB3-7T( = DSM 21699T = NCAIM B 02336T).