Corynebacterium frankenforstense sp. nov. and Corynebacterium lactis sp. nov., isolated from raw cow milk

Author:

Wiertz Raika1,Schulz Stefanie Christine1,Müller Ute2,Kämpfer Peter3,Lipski André1

Affiliation:

1. Rheinische Friedrich-Wilhelms-Universität Bonn, Institut für Ernährungs- und Lebensmittelwissenschaften, Abteilung Lebensmittelmikrobiologie und -hygiene, Meckenheimer Allee 168, 53115 Bonn, Germany

2. Rheinische Friedrich-Wilhelms-Universität Bonn, Institut für Tierwissenschaften, Abteilung Physiologie und Hygiene, Katzenburgweg 7-9, 53115 Bonn, Germany

3. Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, 35392 Giessen, Germany

Abstract

Two groups of Gram-stain positive, aerobic bacterial strains were isolated from raw cow’s milk, from a milking machine and from bulk tank milk. Based on their 16S rRNA gene sequences these isolates formed two distinct groups within the genus Corynebacterium . The sequence similarities of the isolates to the type strains of species of the genus Corynebacterium were below 98.4 %. The presence of menaquinones MK-8(H2) and MK-9(H2), the predominant fatty acid 18 : 1 cis 9 and a polar lipid pattern with several phospholipids but without aminolipids was in accord with the characteristics of this genus. The results of DNA–DNA hybridization, biochemical tests and chemotaxonomic properties allowed genotypic and phenotypic differentiation of the strains from all known species of the genus Corynebacterium . Therefore, the isolates were assigned to two novel species of this genus for which the names Corynebacterium frankenforstense sp. nov. (type strain ST18T = DSM 45800T = CCUG 63371T), and Corynebacterium lactis sp. nov. (type strain RW2-5T = DSM 45799T = CCUG 63372T) are proposed, respectively.

Publisher

Microbiology Society

Subject

General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology

Reference33 articles.

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2. Genus I. Corynebacterium Lehmann and Neumann 1896, 350AL emend. Bernard, Wiebke, Burdz, Reimer, Ng, Singh, Schindle and Pacheo 2010, 877;Bernard,2012

3. Screening for genetic diversity of isolates of anaerobic Fe(II)-oxidizing bacteria using DGGE and whole-cell hybridization;Buchholz-Cleven;Syst Appl Microbiol,1997

4. Nonstaining (KOH) method for determination of gram reactions of marine bacteria;Buck;Appl Environ Microbiol,1982

5. Corynebacterium amycolatum sp. nov. a new mycolic acid-less Corynebacterium species from human skin;Collins;FEMS Microbiol Lett,1988

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