Affiliation:
1. Biology Department, Lycoming College, Williamsport, PA 17701, USA
Abstract
While characterizing a related strain, it was noted that there was little difference between the 16S rRNA gene sequences of
Bacillus indicus
LMG 22858T and
Bacillus cibi
DSM 16189T. Phenotypic characterization revealed differences only in the utilization of mannose and galactose and slight variation in pigmentation. Whole genome shotgun sequencing and comparative genomics were used to calculate established phylogenomic metrics and explain phenotypic differences. The full, genome-derived 16S rRNA gene sequences were 99.74 % similar. The average nucleotide identity (ANI) of the two strains was 98.0 %, the average amino acid identity (AAI) was 98.3 %, and the estimated DNA–DNA hybridization determined by the genome–genome distance calculator was 80.3 %. These values are higher than the species thresholds for these metrics, which are 95 %, 95 % and 70 %, respectively, suggesting that these two strains should be classified as members of the same species. We propose reclassification of
Bacillus cibi
as a later heterotypic synonym of
Bacillus indicus
and an emended description of
Bacillus indicus
.
Funder
US National Science Foundation
Lycoming College
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
21 articles.
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