Effluviibacter roseus gen. nov., sp. nov., isolated from muddy water, belonging to the family ‘Flexibacteraceae’

Abstract

A Gram-negative bacterial isolate (designated SRC-1T) was isolated from an occasional drainage system and characterized by a polyphasic approach to determine its taxonomic position. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain SRC-1T with the family ‘Flexibacteraceae’ of the phylum Bacteroidetes. It showed greatest sequence similarity to Pontibacter actiniarum KMM 6156T (95.5 %) followed by Adhaeribacter aquaticus MBRG1.5T (89.0 %) and Hymenobacter roseosalivarius DSM 11622T (88.9 %), but it differed from these micro-organisms in many phenotypic characteristics. Strain SRC-1T was an obligate aerobe and its cells were non-motile, irregular rods. The major fatty acids included mainly unsaturated and hydroxy fatty acids, including 17 : 1 iso I/anteiso B (36.7 %), 15 : 0 iso (15.8 %) and 17 : 0 iso 3-OH (10.3 %), and the DNA G+C content was 59.5 mol%. From the phenotypic and genotypic analyses it was clear that strain SRC-1T was quite different from members other genera in the family ‘Flexibacteraceae’. Therefore we conclude that strain SRC-1T represents a novel genus, for which the name Effluviibacter gen. nov., containing a single species Effluviibacter roseus sp. nov., is proposed. The type species of the genus is Effluviibacter roseus, the type strain of which is strain SRC-1T (=MTCC 7260T=DSM 17521T).