Aeromonas fluvialis sp. nov., isolated from a Spanish river

Abstract

A Gram-stain-negative, facultatively anaerobic bacterial strain, designated 717T, was isolated from a water sample collected from the Muga river, Girona, north-east Spain. Preliminary analysis of the 16S rRNA gene sequence showed that this strain belonged to the genus Aeromonas, the nearest species being Aeromonas veronii (99.5 % similarity, with seven different nucleotides). A polyphasic study based on a multilocus phylogenetic analysis of five housekeeping genes (gyrB, rpoD, recA, dnaJ and gyrA; 3684 bp) showed isolate 717T to be an independent phylogenetic line, with Aeromonas sobria, Aeromonas veronii and Aeromonas allosaccharophila as the closest neighbour species. DNA–DNA reassociation experiments and phenotypic analysis identified that strain 717T represents a novel species, for which the name Aeromonas fluvialis sp. nov. is proposed, with type strain 717T (=CECT 7401T =LMG 24681T).