Pseudoalteromonas lipolytica sp. nov., isolated from the Yangtze River estuary

Abstract

A strictly aerobic, Gram-negative, non-pigmented bacterial strain, designated LMEB 39T, was isolated from a seawater sample collected from the Yangtze River estuary near the East China Sea and was examined physiologically, chemotaxonomically and phylogenetically. The novel isolate was motile by a single polar flagellum and positive for nitrate reduction and decomposition of casein, gelatin, Tween 20 and Tween 80, but negative for indole production. Chemotaxonomic analysis revealed ubiquinone-8 as the predominant respiratory quinone and phosphatidylethanolamine and phosphatidylglycerol as major polar lipids. The major fatty acids were C16 : 1ω7c/iso-C15 : 02-OH, C16 : 0, C18 : 1ω7c, C12 : 03-OH, C17 : 1ω8cand C17 : 0. The genomic DNA G+C content was 42.3 mol%. Comparative 16S rRNA gene sequence analysis revealed that the isolate belongs to the genusPseudoalteromonas. Strain LMEB 39Texhibited the closest phylogenetic affinity toPseudoalteromonas byunsanensisJCM 12483T(97.4 % sequence similarity). The DNA–DNA reassociation values between strain LMEB 39TandP. byunsanensisJCM 12483TandPseudoalteromonas undinaDSM 6065T(97.2 % sequence similarity) were 31.7 and 30.3 %, respectively. On the basis of phenotypic and genotypic data, strain LMEB 39Trepresents a novel species of the genusPseudoalteromonas, for which the namePseudoalteromonas lipolyticasp. nov. is proposed; the type strain is LMEB 39T(=CGMCC 1.8499T=JCM 15903T).