Identification of lead (Pb(CH3COO)2)-reducing bacteria using 16S rRNA Gene

Abstract

Abstract In recent years bacteria have been used as bioremediation agents to repair polluted environments. The purpose of this research is to discover the types and capabilities of bacteria in reducing lead (Pb(CH3COO)2) concentration from sediments in Karangsong Coast, Indramayu. The method used is exploratory, and the results are analyzed descriptively. In the molecular identification method, 16S rRNA Gene, a universal primer for a bacterium, is used. Bacterial sequences obtained from GenBank data are analyzed by NCBI sites. The results of this research show that 11 bacterial isolates from porewater sediments have the highest lead ( Pb(CH3COO)2) reduction capability from a concentration of 1.5 mg l−1 to 0.364 mg l−1 with an efficiency value of 75.7%. Four bacterial isolates with the highest density are identical to Albirhodobacter marinus strain N9 (ACC. No. NR 126203.1) with 98% identity value and 100% genetic relationship, Pseudoalteromonas tetraodonis strain NBRC 103034 (ACC. No. NR 114187.1) with 81% identity and 84% genetic relationship, Pseudoalteromonas lipolytica strain LMEB 39 (ACC. No. NR 116629.1) with 98% identity and 96% genetic relationship, Pseudoalteromonas lipolytica strain LMEB 39 (ACC. No. NR 116629.1) 93% identity and 87% genetic relationship.