Leisingera nanhaiensis sp. nov., isolated from marine sediment

Abstract

An aerobic, Gram-staining-negative, motile, rod-shaped bacterium, strain NH52FT, was isolated from a sandy sediment sample taken from the South China Sea. On M2 agar medium (a complex medium), colonies were beige in colour. The isolate showed highest 16S rRNA gene sequence similarities to members of the generaLeisingera(96.7 % similarity),Phaeobacter(95.4–96.0 %) andMarinovum(94.1 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NH52FTformed a distinct cluster withLeisingera methylohalidivoransMB2TandLeisingera aquimarinaLMG 24366T. Optimal growth was observed at pH 7.0-8.5 and 25 °C and the new isolate required the presence of 1–4 % (w/v) NaCl. The major fatty acids were C18 : 1ω7c, C16 : 02-OH, C10 : 03-OH, C12 : 03-OH, C16 : 0and 11-methyl C18 : 1ω7c. The DNA G+C content was 60.5 mol%. The phylogenetic and chemotaxonomic characteristics of strain NH52FTwere similar to those of the genusLeisingera. However, the differences in phenotypic properties and the 16S rRNA gene similarity values demonstrated that the new isolate differed from recognized species of the genusLeisingera. On the basis of phenotypic, chemotaxonomic and phylogenetic data, this organism should be classified as a representative of a novel species in the genusLeisingera, for which the nameLeisingera nanhaiensissp. nov. is proposed. The type strain is NH52FT(=LMG 24841T=CCTCC AB 208316T=MCCC 1A04178T).