Affiliation:
1. State Key Laboratory Breeding Base of Marine Genetic Resources, Fujian Key Laboratory of Marine Genetic Resources, Xiamen 361005, PR China
2. Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources of PR China, Xiamen, PR China
3. School of Advanced Manufacturing, Fuzhou University, Jinjiang 362251, PR China
Abstract
Two Gram-stain-negative, chemoheterotrophic, aerobic bacteria, designated IC7T and JM2-8T, were isolated from seawater of the Yellow Sea of China and rhizosphere soil of mangroves in Xiamen, Fujian, respectively. Phylogenetic analyses based on 16S rRNA gene and genome sequences showed that these two novel strains belonged to the family
Roseobacteraceae
. Strain IC7T formed a coherent lineage within the genus
Pseudodonghicola
, showing 98.05 % 16S rRNA gene sequence similarity to
Pseudodonghicola xiamenensis
Y-2T. Strain JM2-8T was most closely related to members of the genus
Sedimentitalea
, showing 96.51 and 96.73 % 16S rRNA gene sequence similarities to
Sedimentitalea nanhaiensis
NH52FT and
Sedimentitalea todarodis
KHS03T, respectively. The two novel strains contained Q-10 as the major quinone, and phosphatidylethanolamine, aminophospholipid, phosphatidylglycerol and phosphatidylcholine as the principal polar lipids. The main fatty acid of strain IC7T was C19 : 0 cyclo ω8c, while the fatty acid profile JM2-8T was dominated by summed feature 8 containing C18 : 1
ω7c and/or C18 : 1
ω6c. The average nucleotide identity and digital DNA–DNA hybridization values between these two novel isolates and their closely related species were below the cut-off values of 95–96 and 70 %, respectively. The combined genotypic and phenotypic data show that strain IC7T represents a novel species of the genus
Pseudodonghicola
, for which the name Pseudodonghicola flavimaris sp. nov. is proposed, with the type strain IC7T (=MCCC 1A02763T=KCTC 82844T), and strain JM2-8T represents a novel species of the genus
Sedimentitalea
, for which the name Sedimentitalea xiamensis sp. nov. is proposed, with the type strain JM2-8T (=MCCC 1A17756T=KCTC 82846T).
Funder
National Natural Science Foundation of China
Scientific Research Foundation of Third Institute of Oceanography, MNR
National Infrastructure for Microbiological Resources
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology