Pseudoclavibacter chungangensis sp. nov., isolated from activated sludge

Author:

Cho Sung-Lim1,Jung Min Young21,Park Mi-Hak1,Chang Young-Hyo2,Yoon Jung-Hoon2,Myung Soon Chul3,Kim Wonyong1

Affiliation:

1. Department of Microbiology, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea

2. Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea

3. Department of Urology, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea

Abstract

The taxonomic position of a Gram-positive, non-spore-forming strain, designated CAU 59T, from activated sludge was investigated. Colony morphology, biochemical tests and chemotaxonomic investigations revealed that strain CAU 59T possessed the characteristics of the genus Pseudoclavibacter. Comparative 16S rRNA gene sequence analysis showed sequence divergence values between strain CAU 59T and other described Pseudoclavibacter species of more than 3.6 %, and the strain formed a hitherto-unknown subline within the genus Pseudoclavibacter. DNA–DNA hybridization studies showed that strain CAU 59T displayed 20.9 % relatedness to its closest phylogenetic neighbour, Pseudoclavibacter helvolus DSM 20419T. The DNA G+C content was 66.2 mol%. The phenotypic, chemotaxonomic and genotypic data indicated that strain CAU 59T represents a novel species of the genus Pseudoclavibacter, for which the name Pseudoclavibacter chungangensis sp. nov. is proposed. The type strain is CAU 59T (=KCTC 22691T =CCUG 58142T).

Publisher

Microbiology Society

Subject

General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology

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