Affiliation:
1. Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China
2. College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, PR China
Abstract
The taxonomic status of a bacterium, strain DC-8T, isolated from activated sludge, was determined using a polyphasic taxonomic approach. The cells of strain DC-8T were Gram-negative, non-motile, non-spore-forming and rod-shaped. The isolate grew at temperature range of 10–40 °C (optimum 30–35 °C), pH range of 5.0–10.0 (optimum 6.5–8.0) and NaCl concentrations of 0–5 % (optimum 0–1 %). The predominant menaquinone of strain DC-8T was MK-7 and major fatty acids were summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c; 39.7 %), iso-C15 : 0 (33.7 %) and C16 : 0 (5.2 %). The DNA G+C content was 39.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain DC-8T was a member of the genus
Sphingobacterium
. Strain DC-8T shared the highest similarity with
Sphingobacterium siyangense
SY1T (98.4 %),
Sphingobacterium multivorum
IAM 14316T (98.3 %),
Sphingobacterium canadense
CR11T (98.0 %) and
Sphingobacterium detergens
6.2ST (97.9 %) and shared less than 97 % similarity with other members of the genus
Sphingobacterium
. DNA–DNA hybridization experiments showed that the DNA–DNA relatedness values between strain DC-8T and its closest phylogenetic neighbours were below 70 %. Based on the phylogenetic analysis, DNA–DNA hybridization, whole-cell fatty acid composition as well as biochemical characteristics, strain DC-8T was clearly distinguished from all recognized species of the genus
Sphingobacterium
and should be classified as a representative of a novel species of the genus
Sphingobacterium
, for which the name Sphingobacterium caeni sp. nov. is proposed. The type strain is DC-8T ( = CCTCC AB 2012020T = KACC 16850T).
Funder
National Science and Technology Support Plan
Fundamental Research Funds for the Central Universities
Natural Science Foundation of Jiangsu Province
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
27 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献